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	Provedor de dados PAB (1) Autor Alves,Francisco Selmo Fernandes (1) Cardoso,Janaina de Fátima Saraiva (1) Diniz,Bruno Leandro Maranhão (1) Leopoldo,Tadeu Bezerra (1) Paula,Ney Rômulo de Oliveira (1) Pinheiro,Raymundo Rizaldo (1) Porfirio,Kenney de Paiva (1) Rêgo,Wagner Martins Fontes do (1) Mais... Palavra-chave Capra aegagrus hircus (1) Clamidofilose (1) Epidemiologia (1) Manejo reprodutivo (1) Ovis aries (1) Pequenos ruminantes. (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Ano 2016 (1) País Brazil (1) Idioma Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 181 Primeira ... 2 3 4 5 6 7 8 9 10 ... Última Molecular marker heterozygosities and genetic distances as correlates of production traits in F1 bovine crosses Genet. Mol. Biol. Tambasco-Talhari,Daniella; Alencar,Maurício Mello de; Paz,Cláudia Cristina Paro de; Cruz,Geraldo Maria da; Rodrigues,Armando de Andrade; Packer,Irineu Umberto; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida. Several studies have investigated the relationship between heterozygosity, genetic distance and production traits. The objective of the present study was to evaluate the influence of the degree of heterozygosity and genetic distance on growth, carcass and reproductive related features in F1 bovine crosses. We tested 10 polymorphic markers in 330 purebred cattle (Nelore, Canchim, Aberdeen Angus and Simental) and 256 crossbred cattle belonging to four crossbred groups. Individual heterozygosities (Hi) and multilocus genetic similarity (Dm) were estimated and used in correlation analysis against individual phenotypic measurements. Significant (p < 0.05) Hi effects occurred for birth weight, 15 to 18 month weight, hot carcass weight and longissimus rib eye... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine crosses; Molecular markers; Heterozygosity; Genetic distance; Production traits. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200007 Molecular markers as a tool to study genetic resources in oysters ArchiMer Boudry, Pierre. Despite the world economic importance of oyster farming, genetic improvement has not yet had a great impact on these species. Oyster farming is traditionnally based on wild stocks, whose natural populations are often overexploited, and frequently does not fulfil market demande.... Tipo: Text Palavras-chave: Molecular markers; Genetic resources; Oysters. Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/acte-3212.pdf Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves) Genet. Mol. Biol. Faria,Patrícia J.; Miyaki,Cristina Y.. The selection of molecular markers for population studies is an important tool for biodiversity conservation. The family Psittacidae contains many endangered and vulnerable species and we tested three kinds of molecular markers for their potential use in population studies of five psitacid species: 43 hyacinth macaws (Anodorhynchus hyacinthinus), 42 blue-and-yellow macaws (Ara ararauna), 23 red-and-green macaws (Ara chloroptera), 19 red-spectacled amazons (Amazona pretrei); and 18 red-tailed amazons (Amazona brasiliensis). We tested 21 clones from a genomic library of golden conure (Guarouba guarouba) minisatellites and 12 pairs of microsatellite primers developed for the domestic chicken (Gallus gallus) and A. hyacinthinus. We also tested seven... Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; Minisatellites; Molecular markers; Parrots; Population studies. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200007 Molecular markers to study the variability within the Eragrostis curvula complex Phyton Zappacosta,D; Meier,M; Carrera,A; Pacheco,G; Cardone,S; Selva,JP; Echenique,V. Weeping lovegrass is well adapted for forage production and useful for soil conservation in semiarid regions, constituting a morphologically diverse group. Diploid genotypes are unfrequent and reproduce sexually, whereas the tetraploids and plants of higher ploidy levels reproduce by apomixis. In the present work RAPD, AFLP and EST-SSR were used in order to assess the reproductive mode through progeny tests, to determine intracultivar homogeneity or seed purity, to establish genetic relationships among the cultivars within the complex and to characterize the new materials obtained by our group. Eight commercial cultivars and three new plant materials were analyzed. Uniform and variable patterns were observed in progenies of apomictic and sexual plants,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Eragrostis; Molecular markers; Apomixis; Seed purity; Cultivar identification. Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572011000200014 Molecular Tools for Rapid and Accurate Detection of Black Truffle (Tuber melanosporum Vitt.) in Inoculated Nursery Plants and Commercial Plantations in Chile Chilean J. Agric. Res. Cordero,Cecilia; Cáceres,Pablo; González,Gloria; Quiroz,Karla; Carmen,Bravo; Ramírez,Ricardo; Caligari,Peter D.S; Carrasco,Basilio; García-Gonzales,Rolando. Truffle (Tuber melanosporum Vitt.) culture is an agroforestry sector in Chile of increasing interest due to the high prices that truffles fetch in the national market and the recent evidence that its commercial production is possible in Chilean climatic and soil conditions. In this study, the efficiency of three methods of DNA extraction from a mix of 5 g of soil and roots from both nursery and field plants of Quercus ilex L. mycorrhized with T. melanosporum were evaluated, and a simple and reproducible protocol was established. Detection of T. melanosporum was performed by the technique of cleaved amplified polymorphic sequence (CAPS) from amplicons generated with the primers ADL1 (5´-GTAACGATAAAGGCCATCTATAGG-3´) and ADL3 (5´-CGTTTTTCCTGAACTCTTCATCAC-3`),... Tipo: Journal article Palavras-chave: Ectomycorrhizas; Molecular markers; PCR; CAPS; Ectomicorrizas; Marcadores moleculares; PCR; CAPS. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300022 Morphoagronomic and molecular characterization of ornamental passion fruit cultivars PAB Fonseca,Kenia Gracielle da; Faleiro,Fábio Gelape; Junqueira,Nilton Tadeu Vilela; Barth,Mariana; Feldberg,Nelson Pires. Abstract: The objective of this work was to validate the morphoagronomic descriptors used in the protection processes of plant cultivars in Brazil, by characterizing six cultivars of ornamental passion fruit. The BRS Rubiflora, BRS Rosea Púrpura, BRS Céu do Cerrado, BRS Roseflora, BRS Estrela do Cerrado, and BRS Pérola do Cerrado cultivars, 33 morphoagronomic descriptors, and two molecular markers were used. The categorical morphoagronomic descriptors were analyzed by frequency distribution and multivariate analyses. The quantitative morphoagronomic descriptors were subjected to the analysis of variance and to the comparison of the means of each cultivar. The random amplified polymorphic DNA (RAPD) and the inter-simple sequence repeats (ISSR) markers were... Tipo: Info:eu-repo/semantics/article Palavras-chave: Passiflora; Molecular markers; Multivariate analysis; Plant cultivar protection. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001000849 Morphologic and molecular characterization of Myrciaria spp species Rev. Bras. Frutic. Pereira,Márcio; Oliveira,Antonio Luis de; Pereira,Regina Eli de Almeida; Sena,Janete Apparecida Desidério; Costa,Juliana Regina Vieira da; Almeida,Marcílio de; Gonçalves,Antonio Natal. The jabuticaba tree is considered one of the most typical Brazilian fruit trees. However, few studies of this plant are found in the literature and even its botanical classification is very controversial. The present research reports some comparisons between jabuticaba species, using morphologic (organography) and molecular markers techniques. The morphologic characteristics of the plant used as markers were compared with specimens of the herbaria from São Paulo and Minas Gerais States and with the descriptions reported in the literature. Molecular differences between the species were identified by the use of RAPD markers. The experiment was made in Piracicaba, Jaboticabal and Ituverava municipal districts in São Paulo State, Brazil. Morphologic and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Jabuticaba trees; Biological identification; Morphologic markers; Molecular markers; RAPD. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452005000300040 New method for early detection of two random amplified polymorphic DNA (RAPD) groups of Staphylococcus aureus causing bovine mastitis infection in Paraná State, Brazil BABT Gonçalves,Dicezar; Gabriel,Jane Eire; Madeira,Humberto Maciel França; Schühli,Guilherme Schnell e; Vicente,Vânia Aparecida. The aim of this work was to develop a fast and accurate molecular approach to allow early detection of two RAPD groups of S. aureus causing bovine mastitis. Seventy five S. aureus isolates from infected animals were characterized by RAPD. Genomic fragments isolated from the unique bands present in either group were cloned and sequenced. Based on the DNA sequences, specific primers were designed to allow for the simultaneous detection of either group by multiplex PCR of S. aureus DNA isolated from clinical and subclinical bovine mastitis. Results showed that these proposed primers set could be used to detect various clinical and subclinical S. aureus isolates as well as the detection of the microorganism in bulk milk. Their use as a specific method for... Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Molecular markers; Subclinical mastitis; Clinical mastitis; Mastitis detection. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200014 New microsatellite markers developed from an enriched microsatellite common bean library PAB Cardoso,Juliana Morini Kupper; Oblessuc,Paula Rodrigues; Campos,Tatiana de; Sforça,Danilo Augusto; Carbonell,Sérgio Augusto Moraes; Chioratto,Alisson Fernando; Formighieri,Eduardo Fernandes; Souza,Anete Pereira de; Benchimol,Luciana Lasry. The objective of this work was to develop new microsatellite markers in common bean. Ninety nine new microsatelitte loci were developed from a microsatellite enriched library for (CT)8 and (GT)8 motifs, from CAL-143 line. The majority of microsatellite sequences (51%) was related to cellular metabolism. The remaining sequences were associated to transcription functions. Only 17.2% of the sequences presented some level of similarity with other plant species genes. Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Molecular markers; Plant breeding; QTL; SSR. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700019 Nucleotide diversity of a major carotenoid biosynthetic pathway gene in wild and cultivated Solanum (Section Lycopersicon) species Braz. J. Plant Physiol. Araújo,Ana H.; Fonseca,Maria Esther de N.; Boiteux,Leonardo S.. A polymerase chain reaction (PCR)-based approach was employed to isolate putative alleles of the chromoplast-specific lycopene beta-cyclase (CYCB) gene from wild and cultivated tomatoes [Solanum (Section Lycopersicon)]. The objective of this work was to establish an effective PCR protocol by testing DNA samples from distinct germplasm accessions with a primer pair designed to selectively target conserved regions present in the available CYCB sequences. This PCR optimization allowed the amplification of 1219 out 1666 bp of the gene in six taxa: S. cheesmaniae, S. peruvianum, S. neorickii, S. pennellii, S. pimpinellifolium and S. lycopersicum. Sixty-three mutation sites (31 transitions, 18 transversions and 14 single base deletions/insertions) were detected... Tipo: Info:eu-repo/semantics/other Palavras-chave: CYCB; Lycopene; Lycopene-beta-cyclase; Nucleotide diversity; Tomatoes; Molecular markers. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000300007 Optimization of heterologous microsatellites in Piracanjuba PAB Carmo,Fausto Moreira da Silva; Polo,Érico Macedo; Silva,Marcos Antônio da; Yazbeck,Gabriel de Menezes. Abstract: The objective of this work was to evaluate 41 microsatellite markers for heterologous amplifications in piracanjuba (Brycon orbignyanus). Some markers were tested for the first time. Loci were optimized for PCR conditions and applied to a sample of 49 individuals. Thirty-one loci resulted in PCR product formation, whereas ten loci yielded intelligible polymorphic patterns in the evaluated sample and can be used for amplifications in this species. From the evaluated markers, four loci (BoM1, BoM13, Bh6, and Bh16) are valid to be applied in the study of piracanjuba. Tipo: Info:eu-repo/semantics/article Palavras-chave: Brycon orbignyanus; Aquaculture; Hatchery; Molecular markers. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015001201236 PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae) Rev. Bras. entomol. Calado,Daniéla; Navarro-Silva,Mario Antonio; Sallum,Maria Anice Mureb. The aim of the present study was to examine genetic variability in populations of An. cruzii by employing PCR-RAPD and PCR-RFLP markers. All analyses were carried out using individuals of the F1 generation of wild caught females obtained in Santa Catarina State (Florianópolis and São Francisco do Sul), Paraná State (Morretes, Paranaguá and Guaratuba) and São Paulo State (Cananéia). In the PCR-RAPD experiments, seven primers were used for comparisons within and among populations. The restriction profile of the ITS2 including a fragment of both 5.8S and 28S regions of the rDNA was obtained with the enzymes BstUI, HaeIII, TaqI, HhaI, Sau96I, HinfI, HincII and NruI. The PCR-RAPD method detected a large number of polymorphic bands. Genetic distance among... Tipo: Info:eu-repo/semantics/article Palavras-chave: Anophelinae; Genetic variability; ITS2; Molecular markers. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262006000300014 Performance of the double-cross tomato hybrids from a partial diallel Ciência e Agrotecnologia Matos,Rafael de; Resende,Juliano Tadeu Vilela de; Zeist,André Ricardo; Corte,Lígia Erpen-Dalla; Da-Silva,Paulo Roberto; Zeffa,Douglas Mariani. ABSTRACT Knowledge of the combining ability of genotypes, genetic diversity, and heterosis play the paramount role in obtaining double-cross hybrids with high productivity. This study was aimed at evaluating these parameters in tomato genotypes using partial diallel crosses among the commercial single-cross hybrids, which verified the possibility of using double-cross hybrids for commercial purposes. Tomato genotypes included 15 double-cross hybrids, their genitors, and two commercial genotypes as checks. Of the fifteen double-cross hybrids of tomato, two crosses exhibited superiority, such as Aguamiel × Compack and Dominador × Compack, mainly for the total and commercial fruit yields. The phenotypic expression of the trait is controlled by the genes with... Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum lycopersicum; ISSR; Molecular markers; Plant breeding; Heterosis.. Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542021000100205 Pilocarpine content and molecular diversity in jaborandi Scientia Agricola Sandhu,Sardul Singh; Abreu,Ilka Nacif; Colombo,Carlos Augusto; Mazzafera,Paulo. Pilocarpine is an imidazol alkaloid exclusively found in Pilocarpus genus and P. microphyllus accumulates its highest content in the leaves. There is no report in the literature on the variability of the pilocarpine content in this genus. A population of 20 genotypes of P. microphyllus from the state of Maranhão, Brazil, was analyzed for Random Amplification of Polymorphic DNA (RAPD) markers and pilocarpine content. Although it was not possible to establish any correlation between these features, the absence or presence of some markers could indicate in some genotypes a possible association with the content of the alkaloid. Tipo: Info:eu-repo/semantics/article Palavras-chave: RAPD; Pilocarpus jaborandi; Molecular diversity; Molecular markers. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000500010 Plant pre-breeding for increased protein content in soybean Glycine max (L.) Merrill Acta Agron. (Palmira) Silva-Rodrigues,Josiane Isabela da; Antunes-Arruda,Klever Márcio; Deniz-Piovesan,Newton; Gonçalves-de Barros,Everaldo. Abstract The aim of this research were to determine protein content and genetic divergence of soybean genotypes in QTL regions of the trait grain protein content for plant breeding purposes. Twenty-nine soybean genotypes were grown in the field in four environments (Viçosa-MG Dec/2009, Visconde do Rio Branco-MG Feb/2010, São Gotardo-MG Feb/2010, and São Gotardo-MG Oct/2011) using the randomized block design with three replicates. The grain protein content was quantified by the infrared spectrometry method. The genetic divergence was estimated by the analysis of 39 microsatellite markers from QTL regions for the soybean grain protein content. The pairs of genotypes with greater genetic distances and protein contents were as follows: BARC-8/CS3032PTA276-3-4... Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant breeding; Molecular markers; QTL; Soybean genotypes; Protein contein. Ano: 2017 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000400618 Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD PAB Andrade-Rodríguez,María; Villegas-Monter,Angel; Carrillo-Castañeda,Guillermo; García-Velázquez,Armando. The objectives of this work were to evaluate the frequency of polyembryony, and to identify zygotic and nucellar seedlings of Citrus volkameriana using RAPD. Twenty-five polyembryonic and eight monoembryonic seeds were cultivated in vitrofor six months. DNA from seedlings was extracted and used in combination with five RAPD primers to identify zygotic or nucellar origin of the seedlings. Environmental conditions of the year affected significantly (P<0.05) the morphological characteristics of fruitsand the number ofembryos per seed. Polyembryonic seeds ranged from 30.9%, 44.8% to 54.4% over three years. Morphological characteristic was not correlated with polyembryony. In vitro culture enable all embryos of each seed to grow, favoring the percentage of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus volkameriana; Rootstock; Embryo culture; Molecular markers; Polymorphism. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000600006 Polymorphic heterologous microsatellite loci for population genetics studies of the white-faced ibis Plegadis chihi (Vieillot, 1817) (Pelecaniformes, Threskiornithidae) Genet. Mol. Biol. Souza,Andiara Silos Moraes de Castro e; Miño,Carolina Isabel; Lama,Silvia Nassif Del. We screened 44 heterologous microsatellites isolated in species of the families Threskiornithidae, Ciconiidae and Ardeidae for their use in a migratory waterbird, the white-faced ibis Plegadis chihi (Vieillot, 1817) (Threskiornithidae). Of the screened loci, 57% amplified successfully and 24% were polymorphic. In two breeding colonies from southern Brazil (N = 131) we detected 32 alleles (2-10 alleles/ locus). Average He over all loci and colonies was 0.55, and the combined probability of excluding false parents, 98%. There was no departure from HWE in any loci or population. Eru6 and Eru4 loci were in non-random association in the Alvorada colony, and NnNF5 and Eru5 in both populations. AMOVA analysis indicated that most of the genetic diversity was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cross-species amplification; Genetic diversity; Molecular markers; White-faced ibis; SSR. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100010 Polymorphism of the grasshopper Schistocerca pallens (Thunberg) (Orthoptera: Acrididae) and its natural pathogen Metarhizium flavoviride Gams &amp; Rozsypal (Hyphomycetes), revealed by RAPD analysis Anais da SEB Silveira,Eni B.; Al-Janabi,Salah M.; Magalhães,Bonifácio P.; Carvalho,Luiz J.C.B.; Tigano,Myrian S.. The genetic variability of 27 individuals from two distinct populations (Northeast and Central Brazil) of the grasshopper Schistocerca pallens (Thunberg) and eight isolates of its natural pathogen, the fungus Metarhizium flavoviride Gams &amp; Rozsypal, was investigated using RAPD analysis. For the grasshoppers, ten different 10-mer oligonucleotide primers of arbitrary sequence were selected for analysis, resulting in 79 scorable binary characters. The program RAPDDIP applied to S. pallens revealed nucleotide diversity of 2.3 and 2.2% for the populations from Rio Grande do Norte (Northeast Brazil) and Federal District (Central Brazil), respectively. These values indicate the presence of high genetic variability within these populations. Conversely, the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Genetic variability; Entomopathogenic fungus; Biological control; Molecular markers. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000100012 Population structures of Brazilian Tall coconut (Cocos nucifera L.) by microsatellite markers Genet. Mol. Biol. Ribeiro,Francisco E.; Baudouin,Luc; Lebrun,Patricia; Chaves,Lázaro J.; Brondani,Claudio; Zucchi,Maria I.; Vencovsky,Roland. Coconut palms of the Tall group were introduced to Brazil from the Cape Verde Islands in 1553. The present study sought to evaluate the genetic diversity among and within Brazilian Tall coconut populations. Samples were collected of 195 trees from 10 populations. Genetic diversity was accessed by investigating 13 simple sequence repeats (SSR) loci. This provided a total of 68 alleles, ranging from 2 to 13 alleles per locus, with an average of 5.23. The mean values of gene diversity (He) and observed heterozygosity (Ho) were 0.459 and 0.443, respectively. The genetic differentiation among populations was estimated at θ = 0.1600 and the estimated apparent outcrossing rate was t a = 0.92. Estimates of genetic distances between the populations varied from... Tipo: Info:eu-repo/semantics/article Palavras-chave: Coconut; Genetic diversity; Genetic variability; Molecular markers; SSR. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400016 Predicting performance of soybean populations using genetic distances estimated with RAPD markers Genet. Mol. Biol. Barroso,Paulo Augusto Vianna; Geraldi,Isaias Olívio; Vieira,Maria Lúcia Carneiro; Pulcinelli,Carlos Eduardo; Vencovsky,Roland; Dias,Carlos Tadeu dos Santos. In order to verify whether genetic distance (GD) is associated with population mean (PM), genetic variance (GV) and the proportion of superior progenies generated by each cross in advanced generations of selfing (PS), the genetic distances between eight soybean lines (five adapted and three non-adapted) were estimated using 213 polymorphic RAPD markers. The genetic distances were partitioned according to Griffing's Model I Method 4 for diallel analysis, i.e., GDij = GD+ GGDi+ GGDj + SGDij. Phenotypic data were recorded for seed yield and plant height for 25 out of 28 populations of a diallel set derived from the eight soybean lines and evaluated from F2:8 to F2:11 generations. No significant correlation for seed yield was detected between GD and GV, while... Tipo: Info:eu-repo/semantics/article Palavras-chave: Soybean; Genetic distance; Molecular markers; RAPD; Prediction. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300020 Registros recuperados: 181 Primeira ... 2 3 4 5 6 7 8 9 10 ... Última

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